By Dr. Ratna Saxena
Platelets are known to have a role in preventing acute blood loss and repairing vascular walls and adjacent tissues after an injury. During wound, healing platelets are activated. They aggregate to release granules containing growth factors, such as TGF-beta, PDGF, IGF, VEGF, EGF and FGF-2, which stimulate the inflammatory cascade and the healing process. PRP is defined as the plasma fraction of autologous blood and contains concentrated platelets. With the functions of platelets, PRP is now widely used in various clinical scenarios, such as in Orthopaedics, Cosmetology, Ophthalmology and of course Gynaecology.
In Infertility, PRP is used for the treatment of Premature Ovarian Failure, Thin Endometrium, and Poor Ovarian Reserve. Thin endometrium poses a great challenge to IVF experts. PRP Therapy has been used with some success in a few instances along with estrogen replacement with platelets being infused in the endometrium on the tenth day. Some positive cases have been reported following the use of this regime. Platelet-rich plasma is prepared using autologous blood drawn in a syringe prefilled with an anticoagulant and centrifuged to separate blood into three layers; red cells at the bottom, buffy layer and plasma at the top. The plasma and the buffy layer were separated and centrifuged to extract the platelets. Thus the PRP is obtained and used.